Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of A549 using 18987-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 18987-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 18987-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 18987-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 18987-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 18987-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 18987-1-AP (DOCK9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 18987-1-AP
IP result of anti-DOCK9 (IP:18987-1-AP, 4ug; Detection:18987-1-AP 1:800) with mouse brain tissue lysate 4000ug.
IHC staining of human kidney using 18987-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 18987-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 18987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 18987-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18987-1-AP (DOCK9 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 18987-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK9 antibody (18987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DOCK9 Polyclonal antibody

DOCK9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18987-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Zizimin-1, ZIZ1, dedicator of cytokinesis 9, Cdc42 guanine nucleotide exchange factor zizimin-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, human brain tissue, mouse brain tissue, human heart tissue, human placenta tissue, HeLa cells, MCF-7 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human kidney tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

18987-1-AP targets DOCK9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	dedicator of cytokinesis 9
Calculated molecular weight	239 kDa
Observed molecular weight	200-236 kDa
GenBank accession number	NM_015296
Gene Symbol	DOCK9
Gene ID (NCBI)	23348
RRID	AB_10596777
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZ29
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DOCK9 is a guanine nucleotide exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Its overexpression induces filopodia formation.

Protocols

Product Specific Protocols
WB protocol for DOCK9 antibody 18987-1-AP	Download protocol
IHC protocol for DOCK9 antibody 18987-1-AP	Download protocol
IF protocol for DOCK9 antibody 18987-1-AP	Download protocol
IP protocol for DOCK9 antibody 18987-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Commun Biol Brain endothelial cells promote breast cancer cell extravasation to the brain via EGFR-DOCK4-RAC1 signalling Authors - Chiara Galloni View Article
human	WB,IHC,IF	Clin Exp Med DOCK9 as a predictive biomarker linked to angiogenesis and immune response in esophageal squamous cell carcinoma Authors - Yaqiang Pan KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 18987-1-AP

WB analysis using 18987-1-AP

WB analysis of human heart using 18987-1-AP

WB analysis of human placenta using 18987-1-AP

WB analysis of mouse brain using 18987-1-AP

WB analysis of human brain using 18987-1-AP

IP experiment of mouse brain using 18987-1-AP

IHC staining of human kidney using 18987-1-AP