Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 23421-1-AP (same clone as 23421-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 23421-1-AP (same clone as 23421-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 23421-1-AP (same clone as 23421-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 23421-1-AP (same clone as 23421-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 23421-1-AP (same clone as 23421-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 23421-1-AP (same clone as 23421-1-PBS)
IP result of anti-DOCK180 (IP:23421-1-AP, 4ug; Detection:23421-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 23421-1-AP (same clone as 23421-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 23421-1-AP (same clone as 23421-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 23421-1-AP (same clone as 23421-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23421-1-AP (DOCK180 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

DOCK180 Polyclonal antibody, PBS Only

DOCK180 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 23421-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23421-1-PBS targets DOCK180 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18069 Product name: Recombinant human DOCK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1565-1865 aa of BC146857 Sequence: KDLIAWQIPFLAEGIRIHGDKVTEALRPFHERMEACFKQLKEKVEKEYGVRIMPSSLDDRRGSRPRSMVRSFTMPSSSRPLSVASVSSLSSDSTPSRPGSDGFALEPLLPKKMHSRSQDKLDKDDLEKEKKDKKKEKRNSKHQEIFEKEFKPTDISLQQSEAVILSETISPLRPQRPKSQVMNVIGSERRFSVSPSSPSSQQTPPPVTPRAKLSFSMQSSLELNGMTGADVADVPPPLPLKGSVADYGNLMENQDLLGSPTPPPPPPHQRHLPPPLPSKTPPPPPPKTTRKQTSVDSGIVQ 相同性解析による交差性が予測される生物種
Full Name	dedicator of cytokinesis 1
Calculated molecular weight	1865 aa, 215 kDa
Observed molecular weight	215 kDa
GenBank accession number	BC146857
Gene Symbol	DOCK1
Gene ID (NCBI)	1793
RRID	AB_2879276
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14185
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

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サポート

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企業情報

抗体

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 23421-1-AP (same clone as 23421-1-PBS)

WB analysis of MCF-7 using 23421-1-AP (same clone as 23421-1-PBS)

WB analysis of A431 using 23421-1-AP (same clone as 23421-1-PBS)

WB analysis of LNCaP using 23421-1-AP (same clone as 23421-1-PBS)

WB analysis of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

IP experiment of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

IHC staining of human colon using 23421-1-AP (same clone as 23421-1-PBS)