Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 23421-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 23421-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 23421-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 23421-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23421-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 23421-1-AP
IP result of anti-DOCK180 (IP:23421-1-AP, 4ug; Detection:23421-1-AP 1:1000) with HeLa cells lysate 3200ug.
IHC staining of human colon using 23421-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50.
IHC staining of human colon using 23421-1-AP
Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50.
IF Staining of HeLa using 23421-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23421-1-AP (DOCK180 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DOCK180 Polyclonal antibody

DOCK180 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 23421-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ced5, dedicator of cytokinesis 1, DOCK1, DOCK180

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, LNCaP cells, MCF-7 cells, A431 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below

Product Information

23421-1-AP targets DOCK180 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18069 Product name: Recombinant human DOCK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1565-1865 aa of BC146857 Sequence: KDLIAWQIPFLAEGIRIHGDKVTEALRPFHERMEACFKQLKEKVEKEYGVRIMPSSLDDRRGSRPRSMVRSFTMPSSSRPLSVASVSSLSSDSTPSRPGSDGFALEPLLPKKMHSRSQDKLDKDDLEKEKKDKKKEKRNSKHQEIFEKEFKPTDISLQQSEAVILSETISPLRPQRPKSQVMNVIGSERRFSVSPSSPSSQQTPPPVTPRAKLSFSMQSSLELNGMTGADVADVPPPLPLKGSVADYGNLMENQDLLGSPTPPPPPPHQRHLPPPLPSKTPPPPPPKTTRKQTSVDSGIVQ 相同性解析による交差性が予測される生物種
Full Name	dedicator of cytokinesis 1
Calculated molecular weight	1865 aa, 215 kDa
Observed molecular weight	215 kDa
GenBank accession number	BC146857
Gene Symbol	DOCK1
Gene ID (NCBI)	1793
RRID	AB_2879276
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14185
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for DOCK180 antibody 23421-1-AP	Download protocol
IHC protocol for DOCK180 antibody 23421-1-AP	Download protocol
IP protocol for DOCK180 antibody 23421-1-AP	Download protocol
WB protocol for DOCK180 antibody 23421-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Diabetes Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing. Authors - Hua Qu View Article
mouse	WB	Cell Death Dis Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways. Authors - Xujuan Sun View Article
zebrafish	WB	bioRxiv Dock1 acts cell-autonomously in Schwann cells to regulate the development, maintenance, and repair of peripheral myelin Authors - Ryan A Doan View Article
human	WB	Biomaterials Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs Authors - Anna L Kersey View Article
human	WB	Exp Cell Res Acetylation of ELMO1 correlates with Rac1 activity and colorectal cancer progress Authors - Chuangkun Li View Article
mouse	WB	Acta Otolaryngol C1QL1 regulates auditory nerve fibers growth via ELMO1-DOCK180-RAC1 integrin Authors - Minglin Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 23421-1-AP

WB analysis of A431 using 23421-1-AP

WB analysis of MCF-7 using 23421-1-AP

WB analysis of LNCaP using 23421-1-AP

WB analysis of HeLa using 23421-1-AP

IP experiment of HeLa using 23421-1-AP

IHC staining of human colon using 23421-1-AP

IHC staining of human colon using 23421-1-AP

IF Staining of HeLa using 23421-1-AP

DOCK180 Polyclonal antibody

DOCK180 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.

Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.

Dock1 acts cell-autonomously in Schwann cells to regulate the development, maintenance, and repair of peripheral myelin

Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs

Acetylation of ELMO1 correlates with Rac1 activity and colorectal cancer progress

C1QL1 regulates auditory nerve fibers growth via ELMO1-DOCK180-RAC1 integrin