Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells, MCF-7 cells, A431 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
23421-1-AP targets DOCK180 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DOCK180 fusion protein Ag18069 相同性解析による交差性が予測される生物種 |
| Full Name | dedicator of cytokinesis 1 |
| Calculated molecular weight | 1865 aa, 215 kDa |
| Observed molecular weight | 215 kDa |
| GenBank accession number | BC146857 |
| Gene Symbol | DOCK1 |
| Gene ID (NCBI) | 1793 |
| RRID | AB_2879276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14185 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| IHC protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| IF protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| IP protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Diabetes Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing. | ||
Cell Death Dis Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways. | ||
bioRxiv Dock1 acts cell-autonomously in Schwann cells to regulate the development, maintenance, and repair of peripheral myelin | ||
Biomaterials Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs | ||
Exp Cell Res Acetylation of ELMO1 correlates with Rac1 activity and colorectal cancer progress |