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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNMT3B Polyclonal antibody (26971-1-AP)

    WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DNMT3B Polyclonal antibody (26971-1-AP)

    WB analysis using 26971-1-AP (same clone as 26971-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26971-1-AP (DNMT3B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using DNMT3B Polyclonal antibody (26971-1-AP)

    IP experiment of K-562 using 26971-1-AP (same clone as 26971-1-PBS)

    IP result of anti-DNMT3B (IP:26971-1-AP, 4ug; Detection:26971-1-AP 1:5000) with K-562 cells lysate 1800 ug. This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNMT3B Polyclonal antibody (26971-1-AP)

    IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DNMT3B Polyclonal antibody (26971-1-AP)

    IHC staining of mouse brain using 26971-1-AP (same clone as 26971-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DNMT3B Polyclonal antibody (26971-1-AP)

    IHC staining of human breast cancer using 26971-1-AP (same clone as 26971-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DNMT3B Polyclonal antibody (26971-1-AP)

    IHC staining of human breast cancer using 26971-1-AP (same clone as 26971-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNMT3B Polyclonal antibody (26971-1-AP)

    IHC staining of mouse testis using 26971-1-AP (same clone as 26971-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DNMT3B Polyclonal antibody (26971-1-AP)

    IHC staining of mouse testis using 26971-1-AP (same clone as 26971-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26971-1-AP (DNMT3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26971-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DNMT3B Polyclonal antibody, PBS Only

DNMT3B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 26971-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ICF, EC:2.1.1.37, DNMT3, DNMT, DNA (cytosine-5)-methyltransferase 3B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26971-1-PBS targets DNMT3B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25117

Product name: Recombinant human DNMT3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001207055

Sequence: MKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSES

相同性解析による交差性が予測される生物種
Full Name DNA (cytosine-5-)-methyltransferase 3 beta
Calculated molecular weight 95 kDa
Observed molecular weight 85-96 kDa
GenBank accession numberNM_001207055
Gene Symbol DNMT3B
Gene ID (NCBI) 1789
RRIDAB_2880705
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBC3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.

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