ChromoTek DNMT1-Trap Agarose Kit

The DNMT1-Trap Agarose Kit is a ready-to-use reagent for the IP of DNMT1 proteins. It consists of an anti-DNMT1 Nanobody/VHH coupled to agarose beads, along with lysis, wash, and elution buffers to use in the IP process.

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Agarose

Cat no : dtak

Synonyms

DNMT1, DNA methyltransferase, AIM, CXXC9, HSN1E, DNMT, DNMT1 Trap



Product Information

The DNMT1-Trap Agarose Kit is a ready-to-use reagent for the IP of DNMT1 proteins. It consists of an anti-DNMT1 Nanobody/VHH coupled to agarose beads, along with lysis, wash, and elution buffers to use in the IP process.

DescriptionImmunoprecipitation of DNMT1 proteins with anti-DNMT1 Nanobody conjugated to agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, Co-IP
Specificity/TargetDNMT1
Binding Capacity2-3 ug of DNMT1 per 10 uL bead slurry
ConjugateAgarose beads; ~90 um (cross-linked 4% agarose beads)
Elution buffer2x SDS-sample buffer (Lammli)
TypeNanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe DNMT1-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_2631372
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

Component Description
DNMT1-Trap Agarose20 reactions (500 µl)
Lysis bufferOptimized for cytoplasmic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution