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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNLZ Polyclonal antibody (31844-1-AP)

    WB analysis using 31844-1-AP (same clone as 31844-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31844-1-AP (DNLZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31844-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of rat heart using 31844-1-AP (same clone as 31844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31844-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of rat heart using 31844-1-AP (same clone as 31844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31844-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of mouse heart using 31844-1-AP (same clone as 31844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31844-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of mouse heart using 31844-1-AP (same clone as 31844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31844-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DNLZ Polyclonal antibody (31844-1-AP)

    IF Staining of MCF-7 using 31844-1-AP (same clone as 31844-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DNLZ antibody (31844-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31844-1-PBS in a different storage buffer formulation.

DNLZ Polyclonal antibody, PBS Only

DNLZ Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31844-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

mtHsp70-escort protein, Hsp70-escort protein 1, HEP1, DNL-type zinc finger protein, C9orf151

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31844-1-PBS targets DNLZ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37114

Product name: Recombinant human DNLZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-178 aa of BC146915

Sequence: MLRTALRGAPRLLSRVQPRAPCLRRLWGRGARPEVAGRRRAWAWGWRRSSSEQGPGPAAALGRVEAAHYQLVYTCKVCGTRSSKRISKLAYHQGVVIVTCPGCQNHHIIADNLGWFSDLNGKRNIEEILTARGEQVHRVAGEGALELVLEAAGAPTSTAAPEAGEDEGPPSPGKTEPS

相同性解析による交差性が予測される生物種
Full Name DNL-type zinc finger
Calculated molecular weight19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC146915
Gene Symbol DNLZ
Gene ID (NCBI) 728489
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5SXM8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNL-type zinc finger (DNLZ, also known as HEP, ZIM17, TIMM15, and C9orf151) is a nuclear-encoded protein that is translocated to the mitochondria, thus it is located in mitochondrion and nucleoplasm (PMID: 22162012). It may function as a co-chaperone towards HSPA9/mortalin, which enhances HSPA9 solubility and stimulates HSPA9 catalytic activity (PMID: 18632665; 20392697; 21530495).

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