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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNLZ Polyclonal antibody (31844-1-AP)

    WB analysis using 31844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31844-1-AP (DNLZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of rat heart using 31844-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of rat heart using 31844-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of mouse heart using 31844-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DNLZ Polyclonal antibody (31844-1-AP)

    IHC staining of mouse heart using 31844-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DNLZ Polyclonal antibody (31844-1-AP)

    IF Staining of MCF-7 using 31844-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DNLZ antibody (31844-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

DNLZ Polyclonal antibody

DNLZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31844-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

mtHsp70-escort protein, Hsp70-escort protein 1, HEP1, DNL-type zinc finger protein, C9orf151

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRT-4 cells
Positive IHC detected inmouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31844-1-AP targets DNLZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37114

Product name: Recombinant human DNLZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-178 aa of BC146915

Sequence: MLRTALRGAPRLLSRVQPRAPCLRRLWGRGARPEVAGRRRAWAWGWRRSSSEQGPGPAAALGRVEAAHYQLVYTCKVCGTRSSKRISKLAYHQGVVIVTCPGCQNHHIIADNLGWFSDLNGKRNIEEILTARGEQVHRVAGEGALELVLEAAGAPTSTAAPEAGEDEGPPSPGKTEPS

相同性解析による交差性が予測される生物種
Full Name DNL-type zinc finger
Calculated molecular weight19 kDa
Observed molecular weight19 kDa
GenBank accession numberBC146915
Gene Symbol DNLZ
Gene ID (NCBI) 728489
RRIDAB_3670126
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5SXM8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNL-type zinc finger (DNLZ, also known as HEP, ZIM17, TIMM15, and C9orf151) is a nuclear-encoded protein that is translocated to the mitochondria, thus it is located in mitochondrion and nucleoplasm (PMID: 22162012). It may function as a co-chaperone towards HSPA9/mortalin, which enhances HSPA9 solubility and stimulates HSPA9 catalytic activity (PMID: 18632665; 20392697; 21530495).

Protocols

Product Specific Protocols
IF protocol for DNLZ antibody 31844-1-APDownload protocol
IHC protocol for DNLZ antibody 31844-1-APDownload protocol
WB protocol for DNLZ antibody 31844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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