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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DNASE2B Polyclonal antibody (22652-1-AP)

    WB analysis of Jurkat using 22652-1-AP (same clone as 22652-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DNASE2B Polyclonal antibody (22652-1-AP)

    WB analysis using 22652-1-AP (same clone as 22652-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using DNASE2B Polyclonal antibody (22652-1-AP)

    WB analysis of A549 using 22652-1-AP (same clone as 22652-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human tonsillitis using 22652-1-AP (same clone as 22652-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human prostate hyperplasia using 22652-1-AP (same clone as 22652-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human prostate hyperplasia using 22652-1-AP (same clone as 22652-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human tonsillitis using 22652-1-AP (same clone as 22652-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DNASE2B Polyclonal antibody (22652-1-AP)

    IF Staining of A549 using 22652-1-AP (same clone as 22652-1-PBS)

    Immunofluorescent analysis of A549 cells using 22652-1-AP (DNASE2B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22652-1-PBS in a different storage buffer formulation.

DNASE2B Polyclonal antibody, PBS Only

DNASE2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22652-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Deoxyribonuclease 2 beta, Deoxyribonuclease II beta, Deoxyribonuclease-2-beta, DLAD, DNase II beta

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22652-1-PBS targets DNASE2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18246

Product name: Recombinant human DNASE2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-361 aa of BC126136

Sequence: MKHKMMARLLRTSFALLFLGLFGVLGAATISCRNEEGKAVDWFTFYKLPKKQNKESGETGLEYLYLDSTTRSWRKSEQLMNDTKSVLGRTLQQLYEAYASKSNNTAYLIYNDGVPKPVNYSRKYGHTKGLLLWNRVQGFWLIHSIPQFPPIPEEGYDYPPTGRRNGQSGICITFKYNQYEAIDSQLLVCNPNVYSCSIPATFHQELIHMPQLCTRASSSEIPGRLLTTLQSAQGQKFLHFAKSDSFLDDIFAAWMAQRLKTHLLTETWQRKRQELPSNCSLPYHVYNIKAIKLSRHSYFSSYQDHAKWCISQKGTKNRWTCIGDLNRSPHQAFRSGGFICTQNWQIYQAFQGLVLYYESCK

相同性解析による交差性が予測される生物種
Full Name deoxyribonuclease II beta
Calculated molecular weight 361 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC126136
Gene Symbol DNASE2B
Gene ID (NCBI) 58511
RRIDAB_2879144
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8WZ79
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DNASE2B, also known as DLAD (DNase II-like acid DNase) or DNase II beta, is a protein-coding gene located on human chromosome 1p22.3. The gene is positioned head-to-head with the urate oxidase (Uox) pseudogene and consists of 6 exons.

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