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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DNASE2B Polyclonal antibody (22652-1-AP)

    WB analysis of Jurkat using 22652-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DNASE2B Polyclonal antibody (22652-1-AP)

    WB analysis of A549 using 22652-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human tonsillitis using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human prostate hyperplasia using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human prostate hyperplasia using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using DNASE2B Polyclonal antibody (22652-1-AP)

    IHC staining of human tonsillitis using 22652-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DNASE2B Polyclonal antibody (22652-1-AP)

    IF Staining of A549 using 22652-1-AP

    Immunofluorescent analysis of A549 cells using 22652-1-AP (DNASE2B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

DNASE2B Polyclonal antibody

DNASE2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22652-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DNase II like acid DNase, DNase II beta, DLAD, Deoxyribonuclease-2-beta, Deoxyribonuclease II beta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, A549 cells
Positive IHC detected inhuman tonsillitis tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22652-1-AP targets DNASE2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18246

Product name: Recombinant human DNASE2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-57 aa of BC126136

Sequence: MMARLLRTSFALLFLGLFGVLGAATISCRNEEGKAVDWFTFYKLPKKQNKESGETGLEYLYLDSTTRSWRKSEQLMNDTKSVLGRTLQQLYEAYASKSNNTAYLIYNDGVPKPVNYSRKYGHTKGLLLWNRVQGFWLIHSIPQFPPIPEEGYDYPPTGRRNGQSGICITFKYNQYEAIDSQLLVCNPNVYSCSIPATFHQELIHMPQLCTRASSSEIPGRLLTTLQSAQGQKFLHFAKSDSFLDDIFAAWMAQRLKTHLLTETWQRKRQELPSNCSLPYHVYNIKAIKLSRHSYFSSYQDHAKWCISQKGTKNRWTCIGDLNRSPHQAFRSGGFICTQNWQIYQAFQGLVLYYESCK

相同性解析による交差性が予測される生物種
Full Name deoxyribonuclease II beta
Calculated molecular weight 361 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC126136
Gene Symbol DNASE2B
Gene ID (NCBI) 58511
RRIDAB_2879144
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8WZ79
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for DNASE2B antibody 22652-1-APDownload protocol
IHC protocol for DNASE2B antibody 22652-1-APDownload protocol
WB protocol for DNASE2B antibody 22652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Biochem Cell Biol

Heat shock factor 4 regulates lens epithelial cell homeostasis by working with lysosome and anti-apoptosis pathways.

Authors - Xiukun Cui
View Article
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