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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis using 67966-1-Ig (same clone as 67966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis of mouse heart using 67966-1-Ig (same clone as 67966-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis of HeLa using 67966-1-Ig (same clone as 67966-1-PBS)

    WB result of DNAJB4 antibody (67966-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB4 transfected HeLa cells. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IF Staining of A431 using 67966-1-Ig (same clone as 67966-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51770-1

    Cytometric bead array sample test of MP51770-1

    Cytometric bead array sample test of MP51770-1, DNAJB4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67966-1-PBS. Detection antibody: 67966-2-PBS.

  • Cytometric bead array standard curve of MP51770-1

    Cytometric bead array standard curve of MP51770-1

    Cytometric bead array standard curve of MP51770-1, DNAJB4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67966-1-PBS. Detection antibody: 67966-2-PBS. Standard:Ag3756. Range: 3.125-100 ng/mL.

  • Featured Product
  • KD/KO Validated

DNAJB4 Monoclonal antibody, PBS Only (Capture)

DNAJB4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67966-1-PBS
Clone No.1F3H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DjB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67966-1-PBS targets DNAJB4 as part of a matched antibody pair:

MP51770-1: 67966-1-PBS capture and 67966-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3756

Product name: Recombinant human DNAJB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-337 aa of BC034721

Sequence: MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS

相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC034721
Gene Symbol DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2918717
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UDY4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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