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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis using 67966-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis of mouse heart using 67966-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DNAJB4 Monoclonal antibody (67966-1-Ig)

    WB analysis of HeLa using 67966-1-Ig

    WB result of DNAJB4 antibody (67966-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB4 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IHC staining of human pancreas cancer using 67966-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DNAJB4 Monoclonal antibody (67966-1-Ig)

    IF Staining of A431 using 67966-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

DNAJB4 Monoclonal antibody

DNAJB4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67966-1-Ig
Clone No.1F3H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DjB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, mouse heart tissue, HEK-293 cells, Jurkat cells, K-562 cells, pig heart, rabbit heart
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67966-1-Ig targets DNAJB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

Tested Reactivity human, mouse, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3756

Product name: Recombinant human DNAJB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-337 aa of BC034721

Sequence: MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS

相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 4
Calculated molecular weight 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC034721
Gene Symbol DNAJB4
Gene ID (NCBI) 11080
RRIDAB_2918717
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UDY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for DNAJB4 antibody 67966-1-IgDownload protocol
WB protocol for DNAJB4 antibody 67966-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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