Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-16780 targets DNAJB12 in IHC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10193 Product name: Recombinant human DNAJB12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-248 aa of BC011812 Sequence: AYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHRRVTDHLGVVYYVGDTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDTDMYHRAQKMGTPSCSRLSEVQASLHG 相同性解析による交差性が予測される生物種 |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 12 |
| Calculated molecular weight | 375 aa, 42 kDa |
| Observed molecular weight | 40-42 kDa |
| GenBank accession number | BC011812 |
| Gene Symbol | DNAJB12 |
| Gene ID (NCBI) | 54788 |
| RRID | AB_2934329 |
| Conjugate | Biotin |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXW2 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin DNAJB12 antibody Biotin-16780 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |