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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13174

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL647-13174

    IF Staining of HeLa using CL647-13174

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 DNAJB1 antibody (CL647-13174) at dilution of 1:200.

  • FC experiment of HeLa using CL647-13174

    FC experiment of HeLa using CL647-13174

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human DNAJB1 (CL647-13174) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DNAJB1 Ready-To-Use IHC Kit. DNAJB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13174-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-13174 targets DNAJB1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC019827
Gene symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 DNAJB1 antibody CL647-13174Download protocol
FC protocol for CL Plus 647 DNAJB1 antibody CL647-13174Download protocol
Standard Protocols
Click here to view our Standard Protocols