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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of MCF-7 using 21473-1-AP (same clone as 21473-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21473-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of mouse spleen using 21473-1-AP (same clone as 21473-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21473-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of MCF-7 using 21473-1-AP (same clone as 21473-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21473-1-PBS in a different storage buffer formulation.

DMRTC1B Polyclonal antibody, PBS Only

DMRTC1B Polyclonal Antibody for WB, Indirect ELISA
Cat No. 21473-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21473-1-PBS targets DMRTC1B in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15419

Product name: Recombinant human DMRTC1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-90 aa of BC029799

Sequence: MAAPPKAPIRVRNLTIRAGALTGKENNMLQPETHIFTAPEEGSSQGALLLGQAPEPLSLPCTPVTLEQQLVSPSGDPHRAPALPSICSTL

相同性解析による交差性が予測される生物種
Full Name DMRT-like family C1B
Calculated molecular weight 192 aa, 20 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC029799
Gene Symbol DMRTC1B
Gene ID (NCBI) 728656
RRIDAB_11064607
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5HYR2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DMRTC1B, also named as Doublesex- and mab-3-related transcription factor C1, is a 192 amino acid protein, which belongs to the DMRT family. DMRTC1B is predominantly expressed in kidney, pancreas, ovary and testis. Detected in brain and in many other tissues. DMRTC1B exists two isoform and molecular weight of isoforms are 20 kDa and 13 kDa.

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