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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of MCF-7 using 21473-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of mouse spleen using 21473-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DMRTC1B Polyclonal antibody (21473-1-AP)

    WB analysis of MCF-7 using 21473-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21473-1-AP (DMRTC1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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DMRTC1B Polyclonal antibody

DMRTC1B Polyclonal Antibody for WB, ELISA
Cat No. 21473-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DMRT like family C1B, DMRTC1, DMRTC1B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21473-1-AP targets DMRTC1B in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMRTC1B fusion protein Ag15419 相同性解析による交差性が予測される生物種
Full Name DMRT-like family C1B
Calculated molecular weight 192 aa, 20 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC029799
Gene Symbol DMRTC1B
Gene ID (NCBI) 728656
RRIDAB_11064607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5HYR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DMRTC1B, also named as Doublesex- and mab-3-related transcription factor C1, is a 192 amino acid protein, which belongs to the DMRT family. DMRTC1B is predominantly expressed in kidney, pancreas, ovary and testis. Detected in brain and in many other tissues. DMRTC1B exists two isoform and molecular weight of isoforms are 20 kDa and 13 kDa.

Protocols

Product Specific Protocols
WB protocol for DMRTC1B antibody 21473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols