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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMPK Polyclonal antibody (16711-1-AP)

    WB analysis using 16711-1-AP (same clone as 16711-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16711-1-AP (DMPK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DMPK Polyclonal antibody (16711-1-AP)

    IHC staining of mouse skeletal muscle using 16711-1-AP (same clone as 16711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DMPK Polyclonal antibody (16711-1-AP)

    IHC staining of mouse skeletal muscle using 16711-1-AP (same clone as 16711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using DMPK Polyclonal antibody (16711-1-AP)

    IF Staining of U-251 using 16711-1-AP (same clone as 16711-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DMPK antibody (16711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HSkMC cells using DMPK Polyclonal antibody (16711-1-AP)

    IF Staining of HSkMC using 16711-1-AP (same clone as 16711-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using DMPK antibody (16711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16711-1-PBS in a different storage buffer formulation.

DMPK Polyclonal antibody, PBS Only

DMPK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16711-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DM, DM kinase, DM1, DM1 protein kinase, DM1PK

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16711-1-PBS targets DMPK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10127

Product name: Recombinant human DMPK protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 306-611 aa of BC062553

Sequence: VPEEARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDFEGATDTCNFDLVEDGLTAMVSGGGETLSDIREGAPLGVHLPFVGYSYSCMALRDSEVPGPTPMELEAEQLLEPHVQAPSLEPSVSPQDETAEVAVPAAVPAAEAEAEVTLRELQEALEEEVLTRQSLSREMEAIRTDNQNFASQLREAEARNRDLEAHVRQLQERMELLQAEGATAVTGVPSPRATDPPSHLDGPPAVAVGQCPLVGPGPMHRRHLLLPARVPRPGLSEALSLLLFAVVLSRAAALGCIGLVA

相同性解析による交差性が予測される生物種
Full Name dystrophia myotonica-protein kinase
Calculated molecular weight 629 aa, 69 kDa
Observed molecular weight 69-75 kDa
GenBank accession numberBC062553
Gene Symbol DMPK
Gene ID (NCBI) 1760
RRIDAB_3741892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09013
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DMPK (Myotonic Dystrophy Protein Kinase) is a serine-threonine protein kinase, strongly linked to myotonic dystrophy type 1 (DM1), the most prevalent muscular dystrophy in adults (PMID: 19309729). It is best known for its association with myotonic dystrophy type 1 (DM1), a multisystemic genetic disorder characterized by myotonia, muscle wasting, cardiac conduction defects, and other systemic manifestations (PMID: 24136222).

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