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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMPK Polyclonal antibody (16711-1-AP)

    WB analysis using 16711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16711-1-AP (DMPK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DMPK Polyclonal antibody (16711-1-AP)

    IHC staining of mouse skeletal muscle using 16711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DMPK Polyclonal antibody (16711-1-AP)

    IHC staining of mouse skeletal muscle using 16711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using DMPK Polyclonal antibody (16711-1-AP)

    IF Staining of U-251 using 16711-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DMPK antibody (16711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

DMPK Polyclonal antibody

DMPK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16711-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DMK, DM1PK, DM1 protein kinase, DM1, DM kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, SH-SY5Y cells, C2C12 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16711-1-AP targets DMPK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMPK fusion protein Ag10127 相同性解析による交差性が予測される生物種
Full Name dystrophia myotonica-protein kinase
Calculated molecular weight 629 aa, 69 kDa
Observed molecular weight 69-75 kDa
GenBank accession numberBC062553
Gene Symbol DMPK
Gene ID (NCBI) 1760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DMPK (Myotonic Dystrophy Protein Kinase) is a serine-threonine protein kinase, strongly linked to myotonic dystrophy type 1 (DM1), the most prevalent muscular dystrophy in adults (PMID: 19309729). It is best known for its association with myotonic dystrophy type 1 (DM1), a multisystemic genetic disorder characterized by myotonia, muscle wasting, cardiac conduction defects, and other systemic manifestations (PMID: 24136222).

Protocols

Product Specific Protocols
WB protocol for DMPK antibody 16711-1-APDownload protocol
IHC protocol for DMPK antibody 16711-1-APDownload protocol
IF protocol for DMPK antibody 16711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols