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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DMPK Polyclonal antibody (16711-1-AP)

    WB analysis using 16711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16711-1-AP (DMPK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DMPK Polyclonal antibody (16711-1-AP)

    IHC staining of mouse skeletal muscle using 16711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DMPK Polyclonal antibody (16711-1-AP)

    IHC staining of mouse skeletal muscle using 16711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16711-1-AP (DMPK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using DMPK Polyclonal antibody (16711-1-AP)

    IF Staining of U-251 using 16711-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DMPK antibody (16711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HSkMC cells using DMPK Polyclonal antibody (16711-1-AP)

    IF Staining of HSkMC using 16711-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using DMPK antibody (16711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

DMPK Polyclonal antibody

DMPK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16711-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DM, DM kinase, DM1, DM1 protein kinase, DM1PK

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, SH-SY5Y cells, C2C12 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells, HSkMC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16711-1-AP targets DMPK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10127

Product name: Recombinant human DMPK protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 306-611 aa of BC062553

Sequence: VPEEARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDFEGATDTCNFDLVEDGLTAMVSGGGETLSDIREGAPLGVHLPFVGYSYSCMALRDSEVPGPTPMELEAEQLLEPHVQAPSLEPSVSPQDETAEVAVPAAVPAAEAEAEVTLRELQEALEEEVLTRQSLSREMEAIRTDNQNFASQLREAEARNRDLEAHVRQLQERMELLQAEGATAVTGVPSPRATDPPSHLDGPPAVAVGQCPLVGPGPMHRRHLLLPARVPRPGLSEALSLLLFAVVLSRAAALGCIGLVA

相同性解析による交差性が予測される生物種
Full Name dystrophia myotonica-protein kinase
Calculated molecular weight 629 aa, 69 kDa
Observed molecular weight 69-75 kDa
GenBank accession numberBC062553
Gene Symbol DMPK
Gene ID (NCBI) 1760
RRIDAB_3741892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ09013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DMPK (Myotonic Dystrophy Protein Kinase) is a serine-threonine protein kinase, strongly linked to myotonic dystrophy type 1 (DM1), the most prevalent muscular dystrophy in adults (PMID: 19309729). It is best known for its association with myotonic dystrophy type 1 (DM1), a multisystemic genetic disorder characterized by myotonia, muscle wasting, cardiac conduction defects, and other systemic manifestations (PMID: 24136222).

Protocols

Product Specific Protocols
IF protocol for DMPK antibody 16711-1-APDownload protocol
IHC protocol for DMPK antibody 16711-1-APDownload protocol
WB protocol for DMPK antibody 16711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Genomics Proteomics

Integrated Proteomics Reveal ALDH1A1 as an Oncogenic Driver and Regulatory Hub in Hepatocellular Carcinoma.

Authors - HANXUN YUE
View Article
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