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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DIS3 Recombinant monoclonal antibody (85705-2-RR)

    WB analysis using 85705-2-RR (same clone as 85705-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85705-2-RR (DIS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85705-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DIS3 Recombinant monoclonal antibody (85705-2-RR)

    IHC staining of human ovary cancer using 85705-2-RR (same clone as 85705-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85705-2-RR (DIS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85705-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using DIS3 Recombinant monoclonal antibody (85705-2-RR)

    IHC staining of human ovary cancer using 85705-2-RR (same clone as 85705-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85705-2-RR (DIS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85705-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DIS3 Recombinant monoclonal antibody (85705-2-RR)

    IF Staining of HeLa using 85705-2-RR (same clone as 85705-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (85705-2-RR, Clone: 242934G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85705-2-PBS in a different storage buffer formulation.

DIS3 Recombinant monoclonal antibody, PBS Only

DIS3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85705-2-PBS
Clone No.242934G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DIS 3, Dis-3, dis3p, EC:3.1.13.-, EXOSC11

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85705-2-PBS targets DIS3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag31725

Product name: Recombinant human DIS3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 607-958 aa of NM_014953

Sequence: TTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK

相同性解析による交差性が予測される生物種
Full Name DIS3 mitotic control homolog (S. cerevisiae)
Calculated molecular weight109KD
Observed molecular weight 110 kDa
GenBank accession numberNM_014953
Gene Symbol DIS3
Gene ID (NCBI) 22894
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2L1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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