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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated DIO1 Polyclonal antibody

DIO1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11790

Synonyms

5DI, DIO1, DIOI, ITDI1, TXDI1, Type 1 DI, Type I 5 deiodinase

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-11790

    IF Staining of A549 using CL488-11790

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 DIO1 antibody (CL488-11790) at dilution of 1:200.

Tested Applications

Positive IF detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 11790-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-11790 targets DIO1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIO1 fusion protein Ag2410 相同性解析による交差性が予測される生物種
Full Name deiodinase, iodothyronine, type I
Calculated molecular weight 249 aa, 29 kDa
Observed molecular weight 29 kDa, 13 kDa
GenBank accession numberBC017955
Gene symbol DIO1
Gene ID (NCBI) 1733
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DIO1(Type I iodothyronine deiodinase) is also named as ITDI1, TXDI1,5DI,DIOI and belongs to the iodothyronine deiodinase family.It is a membrane selenoprotein that catalyzes the deiodination of L-thyroxine to the biologically active thyroid hormone 3,3′,5-triiodothyronine.DIO1 is located in liver, kidney, and thyroid,which has both ORD and IRD activities(PMID: 9389495). It has some isoforms produced by alternative splicing with the MW of 29 kDa, 21 kDa, 13 kDa, 23 kDa, 19 kDa, 4 kDa, 13 kDa, 9 kDa and 10 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DIO1 antibody CL488-11790Download protocol
Standard Protocols
Click here to view our Standard Protocols