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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DGKE Polyclonal antibody (11900-1-AP)

    WB analysis using 11900-1-AP (same clone as 11900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11900-1-AP (DGKE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGKE Polyclonal antibody (11900-1-AP)

    IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGKE Polyclonal antibody (11900-1-AP)

    IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGKE Polyclonal antibody (11900-1-AP)

    IHC staining of human colon cancer using 11900-1-AP (same clone as 11900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DGKE Polyclonal antibody (11900-1-AP)

    IF Staining of A431 using 11900-1-AP (same clone as 11900-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKE antibody (11900-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11900-1-PBS in a different storage buffer formulation.

DGKE Polyclonal antibody, PBS Only

DGKE Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11900-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11900-1-PBS targets DGKE in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2512

Product name: Recombinant human DGKE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-177 aa of BC022297

Sequence: MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRYGLRGHSLSQNAPWESGFHRVV

相同性解析による交差性が予測される生物種
Full Name diacylglycerol kinase, epsilon 64kDa
Calculated molecular weight 64 kDa
Observed molecular weight 60-64 kDa
GenBank accession numberBC022297
Gene Symbol DGKE
Gene ID (NCBI) 8526
RRIDAB_2091145
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52429
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DGKE(Diacylglycerol kinase epsilon) is also named as DAGK5 and belongs to the eukaryotic diacylglycerol kinase family.It may be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction(PMID:16689942).When expressed in mammalian cells, DGK-epsilon showed specificity for arachidonyl-containing diacylglycerol.

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