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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DGKE Polyclonal antibody

DGKE Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11900-1-AP

Synonyms

DAG kinase epsilon, DAGK5, DAGK6, DGK, DGK epsilon, DGKE, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon

Validation Data Gallery

Applications:
  • WB analysis using 11900-1-AP

    WB analysis using 11900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11900-1-AP (DGKE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11900-1-AP

    IHC staining of human colon cancer using 11900-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 11900-1-AP

    IHC staining of human colon cancer using 11900-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 11900-1-AP

    IHC staining of human colon cancer using 11900-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11900-1-AP (DGKE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 11900-1-AP

    IF Staining of A431 using 11900-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKE antibody (11900-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11900-1-AP targets DGKE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKE fusion protein Ag2512 相同性解析による交差性が予測される生物種
Full Name diacylglycerol kinase, epsilon 64kDa
Calculated molecular weight 64 kDa
Observed molecular weight 60-64 kDa
GenBank accession numberBC022297
Gene symbol DGKE
Gene ID (NCBI) 8526
RRIDAB_2091145
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGKE(Diacylglycerol kinase epsilon) is also named as DAGK5 and belongs to the eukaryotic diacylglycerol kinase family.It may be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction(PMID:16689942).When expressed in mammalian cells, DGK-epsilon showed specificity for arachidonyl-containing diacylglycerol.

Protocols

Product Specific Protocols
WB protocol for DGKE antibody 11900-1-APDownload protocol
IHC protocol for DGKE antibody 11900-1-APDownload protocol
IF protocol for DGKE antibody 11900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Sci Rep

microRNA regulatory circuits in a mouse model of inherited retinal degeneration.

Authors - Arpad Palfi
View Article
humanWB

Obesity (Silver Spring)

Rutaecarpin reduces lipids by DGKθ-dependent activation of PPARα

Authors - Di Wu
View Article
mouseWB,IF,IP

Biochim Biophys Acta Mol Cell Biol Lipids

Interaction of ROMK2 channel with lipid kinases DGKE and AGK: Potential channel activation by localized anionic lipid synthesis

Authors - Milena Krajewska
View Article