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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DENND2D Polyclonal antibody (33113-1-AP)

    WB analysis using 33113-1-AP (same clone as 33113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33113-1-AP (DENND2D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DENND2D Polyclonal antibody (33113-1-AP)

    IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DENND2D Polyclonal antibody (33113-1-AP)

    IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using DENND2D Polyclonal antibody (33113-1-AP)

    IHC staining of human stomach cancer using 33113-1-AP (same clone as 33113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DENND2D Polyclonal antibody (33113-1-AP)

    IF Staining of A431 using 33113-1-AP (same clone as 33113-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DENND2D antibody (33113-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

DENND2D Polyclonal antibody, PBS Only

DENND2D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 33113-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DENN Domain Containing 2D , DENN domain-containing protein 2D

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33113-1-PBS targets DENND2D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36381

Product name: Recombinant human DENND2D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 305-471 aa of BC004557

Sequence: FMVGVQMRFQQEVMDSPMEEVLLVNLCEGTFLMSVGDEKDILPPKLQDDILDSLGQGINELKTAEQINEHVSGPFVQFFVKIVGHYASYIKREANGQGHFQERSFCKALTSKTNRRFVKKFVKTQLFSLFIQEAEKSKNPPAGYFQQKILEYEEQKKQKKPREKTVK

相同性解析による交差性が予測される生物種
Full Name DENN/MADD domain containing 2D
Observed molecular weight48-50 kDa
GenBank accession numberBC004557
Gene Symbol DENND2D
Gene ID (NCBI) 79961
RRIDAB_3742860
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H6A0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DENND2D (DENN domain-containing protein 2D) is a protein containing a DENN domain and belongs to the family of membrane transport proteins, participating in intracellular membrane transport and signal transduction processes. The DENND2D protein comprises several conserved domains, such as the uDENN, DENN, and dDENN domains, which are widely present in various signaling proteins. In cancer research, the expression and methylation status of DENND2D hold significant prognostic value. DENND2D may serve as a novel tumor biomarker for predicting cancer progression and recurrence.

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