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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis using 20149-1-AP (same clone as 20149-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis of K-562 using 20149-1-AP (same clone as 20149-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis of Raji using 20149-1-AP (same clone as 20149-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using DDX51 Polyclonal antibody (20149-1-AP)

    WB analysis of Jurkat using 20149-1-AP (same clone as 20149-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using DDX51 Polyclonal antibody (20149-1-AP)

    IP experiment of K-562 using 20149-1-AP (same clone as 20149-1-PBS)

    IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:1000) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 20149-1-PBS in a different storage buffer formulation.

DDX51 Polyclonal antibody, PBS Only

DDX51 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 20149-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20149-1-PBS targets DDX51 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13691

Product name: Recombinant human DDX51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 317-666 aa of BC040185

Sequence: GQKSLAKEQESLVQKTADGYRCLADIVVATPGRLVDHIDQTPGFSLQQLRFLIIDEADRMIDSMHQSWLPRVVAAAFQSEDPADPCALLKRRQAQAVTAASTCCPQMPLQKLLFSATLTQNPEKLQQLGLHQPRLFSTGLAHRGLEDTDGDGDSGKYAFPVGLTHHYVPCSLSSKPLVVLHLVLEMGFSRVLCFTNSRENSHRLFLLVQAFGGVDVAEFSSRYGPGQRRMILKQFEQGKIQLLISTDATARGIDVQGVELVVNYDAPQYLRTYVHRVGRTARAGKTGQAFTLLLKVQERRFLRMLTEAGAPELQRHELSSKLLQPLVPRYEEALSKLEESVKEERKQRAA

相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
Calculated molecular weight 666 aa, 72 kDa
Observed molecular weight 72-80 kDa
GenBank accession numberBC040185
Gene Symbol DDX51
Gene ID (NCBI) 317781
RRIDAB_10665947
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N8A6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The DEAD-box family (DDX) consists of a group of RNA helicases with the activity to hydrolyze ATP and to unwind RNA. The major physiological role of this family was to regulate RNA metabolism including ribosome assembly, RNA splicing, and pre-rRNA processing. DDX51 was a new gene in DDX family composed of 666 amino acids. DDX51 gene was a member of RNA helicases family in charge of regulating RNA metabolism(PMID: 31966432).

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