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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX51 Polyclonal antibody

DDX51 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 20149-1-AP

Synonyms

DDX51, DEAD box protein 51

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 20149-1-AP

    WB analysis of K-562 using 20149-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 20149-1-AP

    WB analysis of Raji using 20149-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 20149-1-AP

    WB analysis of Jurkat using 20149-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 20149-1-AP

    IP experiment of K-562 using 20149-1-AP

    IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:300) with K-562 cells lysate 4000ug.

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, Raji cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 20149-1-AP is DDX51 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX51 fusion protein Ag13691 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
Calculated molecular weight 666 aa, 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC040185
Gene symbol DDX51
Gene ID (NCBI) 317781
RRIDAB_10665947
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DDX51 antibody 20149-1-APDownload protocol
IP protocol for DDX51 antibody 20149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

Authors - Tao Wang
View Article