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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DDX19A Recombinant antibody

DDX19A Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230232B12

Cat no : 83106-1-RR

Synonyms

DDX19 DDX19L, DDX19 like protein, DDX19A, DDX19L, DEAD box protein 19A, DKFZp686C21137, FLJ11126

Validation Data Gallery

Applications:
  • WB analysis using 83106-1-RR

    WB analysis using 83106-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83106-1-RR (DDX19A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 83106-1-RR

    FC experiment of U2OS using 83106-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX19A Recombinant antibody (83106-1-RR, Clone:230232B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, HepG2 cells, mouse thymus tissue
Positive FC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83106-1-RR targets DDX19A in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DDX19A fusion protein Ag7362 相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19A
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC005162
Gene symbol DDX19A
Gene ID (NCBI) 55308
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

Protocols

Product Specific Protocols
WB protocol for DDX19A antibody 83106-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols