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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDX19A Recombinant antibody (83106-1-RR)

    WB analysis using 83106-1-RR (same clone as 83106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83106-1-RR (DDX19A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using DDX19A Recombinant antibody (83106-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83106-1-RR (same clone as 83106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83106-1-RR (DDX19A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using DDX19A Recombinant antibody (83106-1-RR)

    FC experiment of U2OS using 83106-1-RR (same clone as 83106-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX19A Recombinant antibody (83106-1-RR, Clone:230232B12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83106-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83106-1-RR

    Affinity and Kinetic Characterization of 83106-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83106-1-RR against Human DDX19A were performed. The affinity constant is below 1 pM.

DDX19A Recombinant monoclonal antibody, PBS Only

DDX19A Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83106-1-PBS
Clone No.230232B12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

EC:3.6.4.13, DDX19-like protein, DDX19L, DDX19 DDX19L, ATP-dependent RNA helicase DDX19A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83106-1-PBS targets DDX19A in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7362

Product name: Recombinant human DDX19A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 124-478 aa of BC005162

Sequence: MMLAEPPQNLIAQSQSGTGKTAAFVLAMLSRVEPSDRYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

相同性解析による交差性が予測される生物種
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19A
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC005162
Gene Symbol DDX19A
Gene ID (NCBI) 55308
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NUU7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDX19A, also known as DDX19L, belongs to the DEAD-box helicase family. DDX19A was identified as a novel cytosolic RNA sensor that could activate the NLRP3 inflammasome during virus infection. In addition, DDX19A was proven to be associated with NADPH oxidase 1 (NOX1)-mediated oxidative stress in tumor necrosis factor (TNF)-α-induced A549 cell (PMID: 33968728). It may represent biomarkers of metastasis and novel therapeutic targets in CSCC patients.

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