• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DDOST Polyclonal antibody (14916-1-AP)

    WB analysis using 14916-1-AP (same clone as 14916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14916-1-AP (DDOST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DDOST Polyclonal antibody (14916-1-AP)

    WB analysis of HeLa using 14916-1-AP (same clone as 14916-1-PBS)

    WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected HeLa cells. This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using DDOST Polyclonal antibody (14916-1-AP)

    WB analysis of A549 using 14916-1-AP (same clone as 14916-1-PBS)

    WB result of DDOST antibody (14916-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDOST transfected A549 cells. This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse pancreas tissue using DDOST Polyclonal antibody (14916-1-AP)

    IP experiment of mouse pancreas using 14916-1-AP (same clone as 14916-1-PBS)

    IP result of anti-DDOST (IP:14916-1-AP, 4ug; Detection:14916-1-AP 1:1000) with mouse pancreas tissue lysate 3200 ug. This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDOST Polyclonal antibody (14916-1-AP)

    IHC staining of mouse testis using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using DDOST Polyclonal antibody (14916-1-AP)

    IHC staining of mouse testis using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using DDOST Polyclonal antibody (14916-1-AP)

    IHC staining of rat small intestine using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using DDOST Polyclonal antibody (14916-1-AP)

    IHC staining of rat small intestine using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using DDOST Polyclonal antibody (14916-1-AP)

    IHC staining of mouse pancreas using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using DDOST Polyclonal antibody (14916-1-AP)

    IHC staining of mouse pancreas using 14916-1-AP (same clone as 14916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14916-1-AP (DDOST antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14916-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DDOST Polyclonal antibody, PBS Only

DDOST Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14916-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, KIAA0115, OK/SW cl.45, OK/SW-cl.45, OST48

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14916-1-PBS targets DDOST in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6727

Product name: Recombinant human DDOST protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 92-433 aa of BC002594

Sequence: FLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVIQQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAF

相同性解析による交差性が予測される生物種
Full Name dolichyl-diphosphooligosaccharide-protein glycosyltransferase
Calculated molecular weight 49 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002594
Gene Symbol DDOST
Gene ID (NCBI) 1650
RRIDAB_2230534
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP39656
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DDOST (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit), also known as OST48, is an integral membrane protein and a core component of the oligosaccharyltransferase (OST) complex located in the endoplasmic reticulum (ER). It plays a critical role in the transfer of pre-assembled oligosaccharide chains to asparagine residues of nascent polypeptides, a fundamental process known as N-linked protein glycosylation. Beyond its enzymatic function, DDOST contributes to protein folding quality control, endoplasmic reticulum-associated degradation (ERAD), and the unfolded protein response (UPR). Altered expression of DDOST has been implicated in cancer progression, metastasis, and chemoresistance.

||
New chat

Able™

正在加载，请稍候...