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DDOST Polyclonal antibody
DDOST Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14916-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | U-87 MG cells, A549 cells, HeLa cells, mouse pancreas, rat pancreas |
| Positive IP detected in | mouse pancreas tissue |
| Positive IHC detected in | mouse testis tissue, rat small intestine tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14916-1-AP targets DDOST in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DDOST fusion protein Ag6727 相同性解析による交差性が予測される生物種 |
| Full Name | dolichyl-diphosphooligosaccharide-protein glycosyltransferase |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC002594 |
| Gene symbol | DDOST |
| Gene ID (NCBI) | 1650 |
| RRID | AB_2230534 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDOST antibody 14916-1-AP | Download protocol |
| IHC protocol for DDOST antibody 14916-1-AP | Download protocol |
| IP protocol for DDOST antibody 14916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells. | ||
Res Sq Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction | ||
IET Syst Biol An immune-related multi-omics analysis of dolichyl-diphosphooligosaccharide protein glycosyltransferase in glioma: Prognostic value exploration and competitive endogenous RNA network identification | ||
J Hepatocell Carcinoma Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nicole (Verified Customer) (04-28-2022) | 80µl RIPA Hela cell extract: Nitrocellulose: no signal PVDF: weak signal, double band
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