Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
25377-1-PBS targets DDI2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21953 Product name: Recombinant human DDI2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 22-62 aa of BC006011 Sequence: DADFELHNFRALCELESGIPAAESQIVYAERPLTDNHRSLA 相同性解析による交差性が予測される生物種 |
| Full Name | DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae) |
| Calculated molecular weight | 399 aa, 45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC006011 |
| Gene Symbol | DDI2 |
| Gene ID (NCBI) | 84301 |
| RRID | AB_3669472 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TDH0 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
DDI2 contains 399 amino acids and contains an N-terminal ubiquitin-like (UBL) domain and a highly conserved retroviral protease-like (RVP) domain. DDI2 is an aspartic endoprotease that cleaves ubiquitylated target proteins to assist in their processing by the ubiquitin-proteasome system (UPS), especially when the UPS is inhibited(PMID: 32521225).

