Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25377-1-AP targets DDI2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DDI2 fusion protein Ag21953 相同性解析による交差性が予測される生物種 |
| Full Name | DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae) |
| Calculated molecular weight | 399 aa, 45 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC006011 |
| Gene Symbol | DDI2 |
| Gene ID (NCBI) | 84301 |
| RRID | AB_3669472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TDH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDI2 contains 399 amino acids and contains an N-terminal ubiquitin-like (UBL) domain and a highly conserved retroviral protease-like (RVP) domain. DDI2 is an aspartic endoprotease that cleaves ubiquitylated target proteins to assist in their processing by the ubiquitin-proteasome system (UPS), especially when the UPS is inhibited(PMID: 32521225).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDI2 antibody 25377-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |