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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DDI2 Polyclonal antibody (25377-1-AP)

    WB analysis using 25377-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25377-1-AP (DDI2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DDI2 Polyclonal antibody (25377-1-AP)

    IF Staining of HepG2 using 25377-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDI2 antibody (25377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DDI2 Polyclonal antibody

DDI2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25377-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Protein DDI1 homolog 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HL-60 tissue, HUVEC tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25377-1-AP targets DDI2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21953

Product name: Recombinant human DDI2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 22-62 aa of BC006011

Sequence: DADFELHNFRALCELESGIPAAESQIVYAERPLTDNHRSLA

相同性解析による交差性が予測される生物種
Full Name DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae)
Calculated molecular weight 399 aa, 45 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC006011
Gene Symbol DDI2
Gene ID (NCBI) 84301
RRIDAB_3669472
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TDH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDI2 contains 399 amino acids and contains an N-terminal ubiquitin-like (UBL) domain and a highly conserved retroviral protease-like (RVP) domain. DDI2 is an aspartic endoprotease that cleaves ubiquitylated target proteins to assist in their processing by the ubiquitin-proteasome system (UPS), especially when the UPS is inhibited(PMID: 32521225).

Protocols

Product Specific Protocols
WB protocol for DDI2 antibody 25377-1-APDownload protocol
IF protocol for DDI2 antibody 25377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols