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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of A375 using 68114-1-Ig (same clone as 68114-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of HL-60 using 68114-1-Ig (same clone as 68114-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig skin tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of pig skin using 68114-1-Ig (same clone as 68114-1-PBS)

    pig skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of mouse testis using 68114-1-Ig (same clone as 68114-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of mouse skin using 68114-1-Ig (same clone as 68114-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DCT Monoclonal antibody (68114-1-Ig)

    IF Staining of A549 using 68114-1-Ig (same clone as 68114-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68114-1-PBS in a different storage buffer formulation.

DCT Monoclonal antibody, PBS Only

DCT Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68114-1-PBS
Clone No.1B3D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

1B3D4, DT, EC:5.3.3.12, L-dopachrome Delta-isomerase, L-dopachrome tautomerase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68114-1-PBS targets DCT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26165

Product name: Recombinant human DCT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC028311

Sequence: MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNA

相同性解析による交差性が予測される生物種
Full Name dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Calculated molecular weight 519 aa, 59 kDa
Observed molecular weight55-63 kDa
GenBank accession numberBC028311
Gene Symbol DCT
Gene ID (NCBI) 1638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP40126
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

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