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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of A375 using 68114-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of HL-60 using 68114-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig skin tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of pig skin using 68114-1-Ig

    pig skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of mouse testis using 68114-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using DCT Monoclonal antibody (68114-1-Ig)

    WB analysis of mouse skin using 68114-1-Ig

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using DCT Monoclonal antibody (68114-1-Ig)

    IF Staining of A549 using 68114-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

DCT Monoclonal antibody

DCT Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68114-1-Ig
Clone No.1B3D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, ELISA

Synonyms

1B3D4, DT, EC:5.3.3.12, L-dopachrome Delta-isomerase, L-dopachrome tautomerase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, HL-60 cells, pig skin tissue, mouse skin tissue, mouse testis tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68114-1-Ig targets DCT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26165

Product name: Recombinant human DCT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC028311

Sequence: MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNA

相同性解析による交差性が予測される生物種
Full Name dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Calculated molecular weight 519 aa, 59 kDa
Observed molecular weight55-63 kDa
GenBank accession numberBC028311
Gene Symbol DCT
Gene ID (NCBI) 1638
RRIDAB_2923643
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP40126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Protocols

Product Specific Protocols
IF protocol for DCT antibody 68114-1-IgDownload protocol
WB protocol for DCT antibody 68114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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