Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 11535-1-AP (same clone as 11535-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
WB analysis of MOVAS using 11535-1-AP (same clone as 11535-1-PBS)
WB result of DCI antibody (11535-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and DCI knockout MOVAS cells. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
WB analysis using 11535-1-AP (same clone as 11535-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
WB analysis of human lung using 11535-1-AP (same clone as 11535-1-PBS)
human lung tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 11535-1-AP (same clone as 11535-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 11535-1-AP (same clone as 11535-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
WB analysis of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 11535-1-AP (same clone as 11535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11535-1-AP (DCI antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
IF Staining of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)
Immunofluorescent analysis of BxPC-3 cells using 11535-1-AP (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 11535-1-AP (same clone as 11535-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DCI antibody (11535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

DCI Polyclonal antibody, PBS Only

DCI Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11535-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

3,2-trans-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, EC:5.3.3.8

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11535-1-PBS targets DCI in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2094 Product name: Recombinant human DCI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC020228 Sequence: MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEEKG 相同性解析による交差性が予測される生物種
Full Name	dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
Calculated molecular weight	302 aa, 33 kDa
Observed molecular weight	31 kDa
GenBank accession number	BC020228
Gene Symbol	DCI
Gene ID (NCBI)	1632
RRID	AB_2089906
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42126
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

WB analysis of MOVAS using 11535-1-AP (same clone as 11535-1-PBS)

WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

WB analysis of human lung using 11535-1-AP (same clone as 11535-1-PBS)

WB analysis of human brain using 11535-1-AP (same clone as 11535-1-PBS)

WB analysis of human kidney using 11535-1-AP (same clone as 11535-1-PBS)

WB analysis of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)

IHC staining of human prostate cancer using 11535-1-AP (same clone as 11535-1-PBS)

IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

IF Staining of BxPC-3 using 11535-1-AP (same clone as 11535-1-PBS)

IF Staining of HEK-293 using 11535-1-AP (same clone as 11535-1-PBS)