• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DCI Polyclonal antibody (11535-1-AP)

    WB analysis using 11535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DCI Polyclonal antibody (11535-1-AP)

    WB analysis using 11535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of human lung using 11535-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of human brain using 11535-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of human kidney using 11535-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using DCI Polyclonal antibody (11535-1-AP)

    WB analysis of BxPC-3 using 11535-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using DCI Polyclonal antibody (11535-1-AP)

    IHC staining of human prostate cancer using 11535-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11535-1-AP (DCI antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DCI Polyclonal antibody (11535-1-AP)

    IHC staining of mouse liver using 11535-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using DCI Polyclonal antibody (11535-1-AP)

    IHC staining of mouse liver using 11535-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using DCI Polyclonal antibody (11535-1-AP)

    IF Staining of BxPC-3 using 11535-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 11535-1-AP (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DCI Polyclonal antibody (11535-1-AP)

    IF Staining of HEK-293 using 11535-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DCI antibody (11535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

DCI Polyclonal antibody

DCI Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11535-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3,2-trans-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, EC:5.3.3.8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, human lung tissue, human brain tissue, human kidney tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, BxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11535-1-AP targets DCI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2094

Product name: Recombinant human DCI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-285 aa of BC020228

Sequence: MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEEKG

相同性解析による交差性が予測される生物種
Full Name dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
Calculated molecular weight 302 aa, 33 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC020228
Gene Symbol DCI
Gene ID (NCBI) 1632
RRIDAB_2089906
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP42126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

Protocols

Product Specific Protocols
IF protocol for DCI antibody 11535-1-APDownload protocol
IHC protocol for DCI antibody 11535-1-APDownload protocol
WB protocol for DCI antibody 11535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation

Authors - Wanxin Guo
View Article
humanWB

Cell

ACSL4 and polyunsaturated lipids support metastatic extravasation and colonization

Authors - Yuqi Wang
View Article
||
New chat Able
正在加载，请稍候...