Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 11535-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of MOVAS using 11535-1-AP
WB result of DCI antibody (11535-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and DCI knockout MOVAS cells.
WB analysis using 11535-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 11535-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11535-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11535-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 11535-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 11535-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11535-1-AP (DCI antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse liver using 11535-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 11535-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of BxPC-3 using 11535-1-AP
Immunofluorescent analysis of BxPC-3 cells using 11535-1-AP (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 11535-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DCI antibody (11535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

DCI Polyclonal antibody

DCI Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11535-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3,2-trans-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, EC:5.3.3.8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, BxPC-3 cells, HEK-293 cells, human brain tissue, human kidney tissue, human lung tissue, MOVAS cells, HeLa cells, mouse liver tissue, rat liver tissue
Positive IHC detected in	mouse liver tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells, BxPC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

11535-1-AP targets DCI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2094 Product name: Recombinant human DCI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC020228 Sequence: MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEEKG 相同性解析による交差性が予測される生物種
Full Name	dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
Calculated molecular weight	302 aa, 33 kDa
Observed molecular weight	31 kDa
GenBank accession number	BC020228
Gene Symbol	DCI
Gene ID (NCBI)	1632
RRID	AB_2089906
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P42126
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

Protocols

Product Specific Protocols
IF protocol for DCI antibody 11535-1-AP	Download protocol
IHC protocol for DCI antibody 11535-1-AP	Download protocol
WB protocol for DCI antibody 11535-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation Authors - Wanxin Guo View Article
human	WB	Cell ACSL4 and polyunsaturated lipids support metastatic extravasation and colonization Authors - Yuqi Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11535-1-AP

WB analysis of MOVAS using 11535-1-AP

WB analysis using 11535-1-AP

WB analysis of human lung using 11535-1-AP

WB analysis of human brain using 11535-1-AP

WB analysis of human kidney using 11535-1-AP

WB analysis of BxPC-3 using 11535-1-AP

IHC staining of human prostate cancer using 11535-1-AP

IHC staining of mouse liver using 11535-1-AP