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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCBLD2 Recombinant antibody (85000-4-RR)

    WB analysis using 85000-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85000-4-RR (DCBLD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of U2OS cells using DCBLD2 Recombinant antibody (85000-4-RR)

    IP experiment of U2OS using 85000-4-RR

    IP result of anti-DCBLD2 (IP:85000-4-RR, 4ug; Detection:85000-4-RR 1:2000) with U2OS cells lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human colon cancer using 85000-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human colon cancer using 85000-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human rectal cancer using 85000-4-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human rectal cancer using 85000-4-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85000-4-RR

    Affinity and Kinetic Characterization of 85000-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85000-4-RR against Human DCBLD2 were performed. The affinity constant is 0.170 nM.

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DCBLD2 Recombinant antibody

DCBLD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 85000-4-RR
Clone No.242604E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, U2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman colon cancer tissue, human rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85000-4-RR targets DCBLD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DCBLD2 fusion protein Ag32823 相同性解析による交差性が予測される生物種
Full Name discoidin, CUB and LCCL domain containing 2
Calculated molecular weight 775 aa, 89 kDa
Observed molecular weight120 kDa
GenBank accession numberBC029658
Gene Symbol DCBLD2
Gene ID (NCBI) 131566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96PD2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).

Protocols

Product Specific Protocols
WB protocol for DCBLD2 antibody 85000-4-RRDownload protocol
IHC protocol for DCBLD2 antibody 85000-4-RRDownload protocol
IP protocol for DCBLD2 antibody 85000-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols