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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DCBLD2 Recombinant antibody (85000-4-RR)

    WB analysis using 85000-4-RR (same clone as 85000-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85000-4-RR (DCBLD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of U2OS cells using DCBLD2 Recombinant antibody (85000-4-RR)

    IP experiment of U2OS using 85000-4-RR (same clone as 85000-4-PBS)

    IP result of anti-DCBLD2 (IP:85000-4-RR, 4ug; Detection:85000-4-RR 1:2000) with U2OS cells lysate 1120 ug. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human rectal cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using DCBLD2 Recombinant antibody (85000-4-RR)

    IHC staining of human rectal cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85000-4-RR

    Affinity and Kinetic Characterization of 85000-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85000-4-RR against Human DCBLD2 were performed. The affinity constant is 0.170 nM.

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DCBLD2 Recombinant antibody, PBS Only

DCBLD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85000-4-PBS
Clone No.242604E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85000-4-PBS targets DCBLD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DCBLD2 fusion protein Ag32823 相同性解析による交差性が予測される生物種
Full Name discoidin, CUB and LCCL domain containing 2
Calculated molecular weight 775 aa, 89 kDa
Observed molecular weight120 kDa
GenBank accession numberBC029658
Gene Symbol DCBLD2
Gene ID (NCBI) 131566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96PD2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).