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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DBP Polyclonal antibody (12662-1-AP)

    WB analysis using 12662-1-AP (same clone as 12662-1-PBS)

    SH-SY5Y cells lysates were subjected to SDS PAGE followed by western blot with 12662-1-AP (DBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12662-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBP Polyclonal antibody (12662-1-AP)

    IHC staining of mouse brain using 12662-1-AP (same clone as 12662-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12662-1-AP (DBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12662-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DBP Polyclonal antibody (12662-1-AP)

    IHC staining of mouse brain using 12662-1-AP (same clone as 12662-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12662-1-AP (DBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12662-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DBP Polyclonal antibody, PBS Only

DBP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12662-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12662-1-PBS targets DBP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3343

Product name: Recombinant human DBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-325 aa of BC011965

Sequence: MARPVSDRTPAPLLLGGPAGTPPGGGALLGLRSLLQGTSKPKEPASCLLKEKERKAALPAATTPGPGLETAGPADAPAGAVVGGGSPRGRPGPVPAPGLLAPLLWERTLPFGDVEYVDLDAFLLEHGLPPSPPPPGGPSPEPSPARTPAPSPGPGSCGSASPRSSPGHAPARAALGTASGHRAGLTSRDTPSPVDPDTVEVLMTFEPDPADLALSSIPGHETFDPRRHRFSEEELKPQPIMKKARKIQVPEEQKDEKYWSRRYKNNEAAKRSRDARRLKENQISVRAAFLEKENALLRQEVVAVRQELSHYRAVLSRYQAQHGAL

相同性解析による交差性が予測される生物種
Full Name D site of albumin promoter (albumin D-box) binding protein
Calculated molecular weight 325 aa, 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC011965
Gene Symbol DBP
Gene ID (NCBI) 1628
RRIDAB_2877870
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10586
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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