Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
12662-1-AP targets DBP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3343 Product name: Recombinant human DBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC011965 Sequence: MARPVSDRTPAPLLLGGPAGTPPGGGALLGLRSLLQGTSKPKEPASCLLKEKERKAALPAATTPGPGLETAGPADAPAGAVVGGGSPRGRPGPVPAPGLLAPLLWERTLPFGDVEYVDLDAFLLEHGLPPSPPPPGGPSPEPSPARTPAPSPGPGSCGSASPRSSPGHAPARAALGTASGHRAGLTSRDTPSPVDPDTVEVLMTFEPDPADLALSSIPGHETFDPRRHRFSEEELKPQPIMKKARKIQVPEEQKDEKYWSRRYKNNEAAKRSRDARRLKENQISVRAAFLEKENALLRQEVVAVRQELSHYRAVLSRYQAQHGAL 相同性解析による交差性が予測される生物種 |
| Full Name | D site of albumin promoter (albumin D-box) binding protein |
| Calculated molecular weight | 325 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC011965 |
| Gene Symbol | DBP |
| Gene ID (NCBI) | 1628 |
| RRID | AB_2877870 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q10586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DBP antibody 12662-1-AP | Download protocol |
| WB protocol for DBP antibody 12662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of β cell function. | ||
Theranostics PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1. | ||
FASEB J Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver. | ||
Am J Physiol Gastrointest Liver Physiol Parenteral nutrition results in peripheral ileal to hepatic circadian discordance in mice | ||
Commun Biol ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude. | ||
Sci Rep Gene expression profiles during tissue remodeling following bladder outlet obstruction.
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