Validation Data Gallery
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Tested Applications
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-86344-3 targets DAZL in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag37074 Product name: Recombinant human DAZL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-295 aa of BC027595 Sequence: QRSYVVPPAYSAVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDRSIQTVVSCLFNPENRLRNSVVTQDDYFKDKRVHHFRRSRAMLKSV 相同性解析による交差性が予測される生物種 |
| Full Name | deleted in azoospermia-like |
| Calculated molecular weight | 295 aa, 33 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC027595 |
| Gene Symbol | DAZL |
| Gene ID (NCBI) | 1618 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92904 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DAZL antibody CL488-86344-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |