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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    WB analysis of MCF-7 using 20849-1-AP (same clone as 20849-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20849-1-AP (DAX-1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP (same clone as 20849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    IF Staining of MCF-7 using 20849-1-AP (same clone as 20849-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 20849-1-AP (DAX-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20849-1-PBS in a different storage buffer formulation.

DAX-1 Polyclonal antibody, PBS Only

DAX-1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20849-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20849-1-PBS targets DAX-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14416

Product name: Recombinant human DAX-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 202-380 aa of BC011564

Sequence: CGEDHPQQGSTLYCVPTSTNQAQAAPEERPRAPWWDTSSGALRPVALKSPQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRNCWASLLMLELAQDRLQFETVEVSEPSMLQKILTTRRRETGGNEPLPVPTLQHHLAPPAEARKVPSASQVQAIKCFLSKCWSLNISTKEYAY

相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 0, group B, member 1
Calculated molecular weight 470 aa, 52 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC011564
Gene Symbol NR0B1
Gene ID (NCBI) 190
RRIDAB_2878751
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51843
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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