• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    WB analysis of MCF-7 using 20849-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20849-1-AP (DAX-1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    IF Staining of MCF-7 using 20849-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 20849-1-AP (DAX-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DAX-1 Polyclonal antibody

DAX-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20849-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AHC, AHCH, AHX, DAX 1, DAX1, DSS, GTD, HHG, NR0B1, NROB1, Nuclear receptor DAX 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20849-1-AP targets DAX-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14416

Product name: Recombinant human DAX-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 202-380 aa of BC011564

Sequence: CGEDHPQQGSTLYCVPTSTNQAQAAPEERPRAPWWDTSSGALRPVALKSPQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRNCWASLLMLELAQDRLQFETVEVSEPSMLQKILTTRRRETGGNEPLPVPTLQHHLAPPAEARKVPSASQVQAIKCFLSKCWSLNISTKEYAY

相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 0, group B, member 1
Calculated molecular weight 470 aa, 52 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC011564
Gene Symbol NR0B1
Gene ID (NCBI) 190
RRIDAB_2878751
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51843
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for DAX-1 antibody 20849-1-APDownload protocol
IHC protocol for DAX-1 antibody 20849-1-APDownload protocol
WB protocol for DAX-1 antibody 20849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...