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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dopamine Transporter/DAT Polyclonal antibody (22524-1-AP)

    WB analysis using 22524-1-AP (same clone as 22524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Dopamine Transporter/DAT Polyclonal antibody (22524-1-AP)

    IP experiment of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IP result of anti-Dopamine Transporter/DAT (IP:22524-1-AP, 4ug; Detection:22524-1-AP 1:1000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Dopamine Transporter/DAT Polyclonal antibody (22524-1-AP)

    IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Dopamine Transporter/DAT Polyclonal antibody (22524-1-AP)

    IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Dopamine Transporter/DAT Polyclonal antibody (22524-1-AP)

    IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Dopamine Transporter/DAT Polyclonal antibody (22524-1-AP)

    IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Dopamine Transporter/DAT Polyclonal antibody (22524-1-AP)

    IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Dopamine Transporter/DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

Dopamine Transporter/DAT Polyclonal antibody, PBS Only

Dopamine Transporter/DAT Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 22524-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

2, SLC6A3, DA transporter, DAT, DAT1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22524-1-PBS targets Dopamine Transporter/DAT in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18297

Product name: Recombinant human SLC6A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-62 aa of BC133003

Sequence: MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRET

相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
Calculated molecular weight 620 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC133003
Gene Symbol DAT
Gene ID (NCBI) 6531
RRIDAB_2879116
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01959
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).

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