Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 52 publications below |
| IHC | See 7 publications below |
| IF | See 18 publications below |
Product Information
22524-1-AP targets Dopamine Transporter/DAT in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18297 Product name: Recombinant human SLC6A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-62 aa of BC133003 Sequence: MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRET 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 |
| Calculated molecular weight | 620 aa, 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC133003 |
| Gene Symbol | DAT |
| Gene ID (NCBI) | 6531 |
| RRID | AB_2879116 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01959 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Dopamine Transporter/DAT antibody 22524-1-AP | Download protocol |
| IP protocol for Dopamine Transporter/DAT antibody 22524-1-AP | Download protocol |
| WB protocol for Dopamine Transporter/DAT antibody 22524-1-AP | Download protocol |
| IF protocol for Dopamine Transporter/DAT antibody 22524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci Propagation of pathologic α-synuclein from kidney to brain may contribute to Parkinson's disease | ||
J Nanobiotechnology Engineered neuronal exosomes mediate α-synuclein clearance to ameliorate Parkinson's disease. | ||
Adv Sci (Weinh) NAD+-Boosters Improve Mitochondria Quality Control In Parkinson's Disease Models Via Mitochondrial UPR | ||
Sci Adv Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits | ||
Sci Adv A nanoparticle-based wireless deep brain stimulation system that reverses Parkinson's disease |