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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated DARS Monoclonal antibody

DARS Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C5D3

Cat no : CL488-67930

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-67930

    IF Staining of HeLa using CL488-67930

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DARS antibody (CL488-67930, Clone: 3C5D3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67930

    FC experiment of HeLa using CL488-67930

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DARS (CL488-67930, Clone:3C5D3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DARS1 Ready-To-Use IHC Kit. DARS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67930-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67930 targets DARS in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DARS fusion protein Ag7059 相同性解析による交差性が予測される生物種
Full Name aspartyl-tRNA synthetase
Calculated molecular weight 57 kDa
Observed molecular weight50-57 kDa
GenBank accession numberBC000629
Gene symbol DARS
Gene ID (NCBI) 1615
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DARS antibody CL488-67930Download protocol
FC protocol for CL Plus 488 DARS antibody CL488-67930Download protocol
Standard Protocols
Click here to view our Standard Protocols