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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis using 20048-1-AP (same clone as 20048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of HeLa using 20048-1-AP (same clone as 20048-1-PBS)

    WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    WB analysis of A431 using 20048-1-AP (same clone as 20048-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DAPK2-Specific Polyclonal antibody (20048-1-AP)

    IF Staining of HepG2 using 20048-1-AP (same clone as 20048-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAPK2-Specific antibody (20048-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DAPK2-Specific Polyclonal antibody, PBS Only

DAPK2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20048-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20048-1-PBS targets DAPK2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name death-associated protein kinase 2
Calculated molecular weight 43 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberNM_014326
Gene Symbol DAPK2
Gene ID (NCBI) 23604
RRIDAB_10667409
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIK4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.

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