Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, HepG2 cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
20048-1-AP targets DAPK2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | death-associated protein kinase 2 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 38-43 kDa |
| GenBank accession number | NM_014326 |
| Gene Symbol | DAPK2 |
| Gene ID (NCBI) | 23604 |
| RRID | AB_10667409 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UIK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DAPK2-Specific antibody 20048-1-AP | Download protocol |
| IHC protocol for DAPK2-Specific antibody 20048-1-AP | Download protocol |
| WB protocol for DAPK2-Specific antibody 20048-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |