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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 24 Polyclonal antibody (33702-1-AP)

    IHC staining of human breast cancer using 33702-1-AP (same clone as 33702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 33702-1-AP (Cytokeratin 24 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33702-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cytokeratin 24 Polyclonal antibody (33702-1-AP)

    IHC staining of human breast cancer using 33702-1-AP (same clone as 33702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 33702-1-AP (Cytokeratin 24 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33702-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 24 Polyclonal antibody (33702-1-AP)

    IF Staining of A431 using 33702-1-AP (same clone as 33702-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 24 antibody (33702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33702-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Cytokeratin 24 Polyclonal antibody (33702-1-AP)

    IF Staining of A431 using 33702-1-AP (same clone as 33702-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 24 antibody (33702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33702-1-PBS in a different storage buffer formulation.

Cytokeratin 24 Polyclonal antibody, PBS Only

Cytokeratin 24 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 33702-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

CK-24, Cytokeratin-24, K24, KA24, Keratin, type I cytoskeletal 24

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33702-1-PBS targets Cytokeratin 24 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag40474

Product name: Recombinant human KRT24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 426-525 aa of BC111968

Sequence: AEYKQLLDIKTRLEVEIETYRRLLDGEGGGSSFAEFGGRNSGSVNMGSRDLVSGDSRSGSCSGQGRDSSKTRVTKTIVEELVDGKVVSSQVSSISEVKVK

相同性解析による交差性が予測される生物種
Full Name keratin 24
GenBank accession numberBC111968
Gene Symbol KRT24
Gene ID (NCBI) 192666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ2M2I5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cytokeratin 24 (CK-24), also known as Keratin 24 (KRT24), is a relatively recently identified type I cytokeratin. It is encoded by the KRT24 gene located on human chromosome 17. KRT24 is strongly expressed in keratinocytes, colon, spleen, and placenta. Previous research has suggested that KRT24 potentially acts as a oncogene in the early stages of colorectal cancer.

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