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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Cytokeratin 19 Polyclonal (CL594-10712)

    IF Staining of HeLa using CL594-10712

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Cytokeratin 19 antibody (CL594-10712) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Cytokeratin 19 Polyclonal (CL594-10712)

    IF Staining of HepG2 using CL594-10712

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Cytokeratin 19 antibody (CL594-10712) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite®594-conjugated Cytokeratin 19 Polyclonal (CL594-10712)

    FC experiment of MCF-7 using CL594-10712

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Cytokeratin 19 (CL594-10712) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-10712
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-10712 targets Cytokeratin 19 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1085

Product name: Recombinant human CK19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 80-400 aa of BC007628

Sequence: EKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL

相同性解析による交差性が予測される生物種
Full Name keratin 19
Calculated molecular weight 40 kDa
GenBank accession numberBC007628
Gene Symbol Cytokeratin 19
Gene ID (NCBI) 3880
RRIDAB_2919743
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP08727
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Protocols

Product Specific Protocols
IF protocol for CL594 Cytokeratin 19 antibody CL594-10712Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biosens Bioelectron

Constructing a highly sensitive duplex immunoassay using AuNPs and AgNPs as nanolabels for investigating the epithelial-mesenchymal transition occurring on circulating tumor cells with lung cancer patients

Authors - Yunuo Dai
View Article