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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Cytokeratin 15 Monoc (CL488-60247)

    IF Staining of HeLa using CL488-60247

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 15 antibody (CL488-60247, Clone: 6B4F8 ) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated Cytokeratin 15 Monoclonal antibody

Cytokeratin 15 Monoclonal Antibody for IF/ICC
Cat No. CL488-60247
Clone No.6B4F8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

KRT15, Cytokeratin-15 , Cytokeratin,Keratin, CK-15, CK15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60247 targets Cytokeratin 15 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 15 fusion protein Ag0185 相同性解析による交差性が予測される生物種
Full Name keratin 15
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002641
Gene Symbol KRT15
Gene ID (NCBI) 3866
RRIDAB_3672865
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19012
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 15 antibody CL488-60247Download protocol
Standard Protocols
Click here to view our Standard Protocols