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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-10143

Synonyms

CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

Validation Data Gallery

Applications:
  • FC experiment of A431 using CL647-10143

    FC experiment of A431 using CL647-10143

    1X10^6 A431 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cytokeratin 14 (CL647-10143) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10143-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-10143 targets Cytokeratin 14 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 14 fusion protein Ag0188 相同性解析による交差性が予測される生物種
Full Name keratin 14
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC002690
Gene symbol KRT14
Gene ID (NCBI) 3861
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 Cytokeratin 14 antibody CL647-10143Download protocol
Standard Protocols
Click here to view our Standard Protocols